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FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
PAM: a framework for integrated analysis of imaging, single-molecule, and ensemble fluorescence data
W Schrimpf, A Barth, J Hendrix, DC Lamb - Biophysical journal, 2018 - cell.com
Fluorescence microscopy and spectroscopy data hold a wealth of information on the
investigated molecules, structures, or organisms. Nowadays, the same fluorescence data set …
investigated molecules, structures, or organisms. Nowadays, the same fluorescence data set …
Identifying molecular dynamics in single-molecule FRET experiments with burst variance analysis
Histograms of single-molecule Förster resonance energy transfer (FRET) efficiency are often
used to study the structures of biomolecules and relate these structures to function. Methods …
used to study the structures of biomolecules and relate these structures to function. Methods …
FRETBursts: an open source toolkit for analysis of freely-diffusing single-molecule FRET
Single-molecule Förster Resonance Energy Transfer (smFRET) allows probing
intermolecular interactions and conformational changes in biomacromolecules, and …
intermolecular interactions and conformational changes in biomacromolecules, and …
Disentangling subpopulations in single-molecule FRET and ALEX experiments with photon distribution analysis
Among the advantages of the single-molecule approach when used to study biomolecular
structural dynamics and interaction is its ability to distinguish between and independently …
structural dynamics and interaction is its ability to distinguish between and independently …
Shrinking gate fluorescence correlation spectroscopy yields equilibrium constants and separates photophysics from structural dynamics
Fluorescence correlation spectroscopy is a versatile tool for studying fast conformational
changes of biomolecules especially when combined with Förster resonance energy transfer …
changes of biomolecules especially when combined with Förster resonance energy transfer …
Rapid, solution-based characterization of optimized SERS nanoparticle substrates
We demonstrate the rapid optical characterization of large numbers of individual metal
nanoparticles freely diffusing in colloidal solution by confocal laser spectroscopy to guide …
nanoparticles freely diffusing in colloidal solution by confocal laser spectroscopy to guide …
[HTML][HTML] Single-particle tracking reveals anti-persistent subdiffusion in cell extracts
K Speckner, M Weiss - Entropy, 2021 - mdpi.com
Single-particle tracking (SPT) has become a powerful tool to quantify transport phenomena
in complex media with unprecedented detail. Based on the reconstruction of individual …
in complex media with unprecedented detail. Based on the reconstruction of individual …
[HTML][HTML] Unraveling multi-state molecular dynamics in single-molecule FRET experiments. II. Quantitative analysis of multi-state kinetic networks
O Opanasyuk, A Barth, TO Peulen… - The Journal of …, 2022 - pubs.aip.org
Single-molecule Förster Resonance Energy Transfer (smFRET) experiments are ideally
suited to resolve the structural dynamics of biomolecules. A significant challenge to date is …
suited to resolve the structural dynamics of biomolecules. A significant challenge to date is …
Characterizing Single‐Molecule FRET Dynamics with Probability Distribution Analysis
Probability distribution analysis (PDA) is a recently developed statistical tool for predicting
the shapes of single‐molecule fluorescence resonance energy transfer (smFRET) …
the shapes of single‐molecule fluorescence resonance energy transfer (smFRET) …