PAM: a framework for integrated analysis of imaging, single-molecule, and ensemble fluorescence data

W Schrimpf, A Barth, J Hendrix, DC Lamb - Biophysical journal, 2018 - cell.com
Fluorescence microscopy and spectroscopy data hold a wealth of information on the
investigated molecules, structures, or organisms. Nowadays, the same fluorescence data set …

Identifying molecular dynamics in single-molecule FRET experiments with burst variance analysis

JP Torella, SJ Holden, Y Santoso, J Hohlbein… - Biophysical journal, 2011 - cell.com
Histograms of single-molecule Förster resonance energy transfer (FRET) efficiency are often
used to study the structures of biomolecules and relate these structures to function. Methods …

Disentangling subpopulations in single-molecule FRET and ALEX experiments with photon distribution analysis

TE Tomov, R Tsukanov, R Masoud, M Liber… - Biophysical journal, 2012 - cell.com
Among the advantages of the single-molecule approach when used to study biomolecular
structural dynamics and interaction is its ability to distinguish between and independently …

Shrinking gate fluorescence correlation spectroscopy yields equilibrium constants and separates photophysics from structural dynamics

T Schröder, J Bohlen, SE Ochmann, P Schüler… - Proceedings of the …, 2023 - pnas.org
Fluorescence correlation spectroscopy is a versatile tool for studying fast conformational
changes of biomolecules especially when combined with Förster resonance energy transfer …

Rapid, solution-based characterization of optimized SERS nanoparticle substrates

TA Laurence, G Braun, C Talley… - Journal of the …, 2009 - ACS Publications
We demonstrate the rapid optical characterization of large numbers of individual metal
nanoparticles freely diffusing in colloidal solution by confocal laser spectroscopy to guide …

[HTML][HTML] Single-particle tracking reveals anti-persistent subdiffusion in cell extracts

K Speckner, M Weiss - Entropy, 2021 - mdpi.com
Single-particle tracking (SPT) has become a powerful tool to quantify transport phenomena
in complex media with unprecedented detail. Based on the reconstruction of individual …

[HTML][HTML] Unraveling multi-state molecular dynamics in single-molecule FRET experiments. II. Quantitative analysis of multi-state kinetic networks

O Opanasyuk, A Barth, TO Peulen… - The Journal of …, 2022 - pubs.aip.org
Single-molecule Förster Resonance Energy Transfer (smFRET) experiments are ideally
suited to resolve the structural dynamics of biomolecules. A significant challenge to date is …

Characterizing Single‐Molecule FRET Dynamics with Probability Distribution Analysis

Y Santoso, JP Torella, AN Kapanidis - ChemPhysChem, 2010 - Wiley Online Library
Probability distribution analysis (PDA) is a recently developed statistical tool for predicting
the shapes of single‐molecule fluorescence resonance energy transfer (smFRET) …