Synthetic biology for plant genetic engineering and molecular farming

Y Wang, GS Demirer - Trends in Biotechnology, 2023 - cell.com
Many efforts have been put into engineering plants to improve crop yields and stress
tolerance and boost the bioproduction of valuable molecules. Yet, our capabilities are still …

Plant protoplasts in the age of synthetic biology

I Reyna-Llorens, M Ferro-Costa… - Journal of experimental …, 2023 - academic.oup.com
Protoplasts, which are plant cells with their cell walls removed, have been used for decades
in plant research and have been instrumental in genetic transformation and the study of …

Phytosensors: harnessing plants to understand the world around us

AC Pfotenhauer, SC Lenaghan - Current Opinion in Biotechnology, 2024 - Elsevier
Highlights•Review of the state of the art approaches for current phytosensor
designs.•Analysis of the future design space for phytosensors.•Comparison of strengths and …

Designed to breathe: synthetic biology applications in plant hypoxia

M Lavilla-Puerta, B Giuntoli - Plant Physiology, 2025 - academic.oup.com
Over the past years, plant hypoxia research has produced a considerable number of new
resources to monitor low oxygen responses in model species, mainly Arabidopsis thaliana …

Performance of abiotic stress-inducible synthetic promoters in genetically engineered hybrid poplar (Populus tremula × Populus alba)

Y Yang, Y Shao, TA Chaffin, JH Lee… - Frontiers in Plant …, 2022 - frontiersin.org
Abiotic stresses can cause significant damage to plants. For sustainable bioenergy crop
production, it is critical to generate resistant crops to such stress. Engineering promoters to …

An Optimized Version of the Small Synthetic Genome (Mini-Synplastome) for Plastid Metabolic Engineering in Solanum tuberosum (Potato)

A Occhialini, G King, M Majdi… - ACS Synthetic …, 2024 - ACS Publications
Plastids represent promising targets in plant genetic engineering for many biotech
applications, ranging from their use as bioreactors for the overproduction of valuable …

Construction of viral-based expression vectors for high-level production of human interferon alpha 2b in plants

Y Sindarovska, M Kuchuk - Applied Microbiology and Biotechnology, 2024 - Springer
Human interferon (hINF) alpha 2b is clinically important pharmaceutical product included in
combinatory therapy against chronic hepatitis C and B and complex therapy against several …

A high-efficient protoplast transient system for screening gene editing elements in Salvia miltiorrhiza

J Shao, B Peng, Y Zhang, X Yan, X Yao, X Hu, L Li… - Plant Cell Reports, 2024 - Springer
Key message A high-efficiency protoplast transient system was devised to screen genome
editing elements in Salvia miltiorrhiza. Abstract Medicinal plants with high-value …

Development of new binary expression systems for plant synthetic biology

AC Pfotenhauer, DN Reuter, M Clark, SA Harbison… - Plant Cell Reports, 2024 - Springer
Key message A novel plant binary expression system was developed from the compactin
biosynthetic pathway 27 of Penicillium citrinum ML-236B. The system achieved> fivefold …

The Genetic Engineering Toolbox for Transformation of Higher Plant Plastids

A Occhialini - … Gene Expression: Regulation, Stress Signaling and …, 2024 - Springer
Plastids are membrane-bound organelles that assume a wide range of essential functions in
the plant kingdom, ranging from photosynthesis to coordinating with other compartments in …