[HTML][HTML] 3D printing of hydrogels: Rational design strategies and emerging biomedical applications

J Li, C Wu, PK Chu, M Gelinsky - Materials Science and Engineering: R …, 2020 - Elsevier
Abstract 3D printing alias additive manufacturing can transform 3D virtual models created by
computer-aided design (CAD) into physical 3D objects in a layer-by-layer manner …

The tubulin code and its role in controlling microtubule properties and functions

C Janke, MM Magiera - Nature Reviews Molecular Cell Biology, 2020 - nature.com
Microtubules are core components of the eukaryotic cytoskeleton with essential roles in cell
division, sha**, motility and intracellular transport. Despite their functional heterogeneity …

In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains

H Van den Hoek, N Klena, MA Jordan, G Alvarez Viar… - Science, 2022 - science.org
The cilium is an antenna-like organelle that performs numerous cellular functions, including
motility, sensing, and signaling. The base of the cilium contains a selective barrier that …

Stimulated emission depletion microscopy

G Lukinavičius, J Alvelid, R Gerasimaitė… - Nature Reviews …, 2024 - nature.com
In this Primer, we focus on the most recent advancements in stimulated emission depletion
(STED) microscopy, encompassing optics, computational microscopy and probes design …

iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy

V Louvel, R Haase, O Mercey, MH Laporte… - Nature …, 2023 - nature.com
Expansion microscopy (ExM) is a highly effective technique for super-resolution
fluorescence microscopy that enables imaging of biological samples beyond the diffraction …

Current progress in expansion microscopy: chemical strategies and applications

G Wen, V Leen, T Rohand, M Sauer… - Chemical …, 2023 - ACS Publications
Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

Nuclear pores as versatile reference standards for quantitative superresolution microscopy

JV Thevathasan, M Kahnwald, K Cieśliński, P Hoess… - Nature …, 2019 - nature.com
Quantitative fluorescence and superresolution microscopy are often limited by insufficient
data quality or artifacts. In this context, it is essential to have biologically relevant control …

[HTML][HTML] Time-series reconstruction of the molecular architecture of human centriole assembly

MH Laporte, D Gambarotto, É Bertiaux, L Bournonville… - Cell, 2024 - cell.com
Centriole biogenesis, as in most organelle assemblies, involves the sequential recruitment
of sub-structural elements that will support its function. To uncover this process, we …

Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy

D Sarkar, J Kang, AT Wassie, ME Schroeder… - Nature Biomedical …, 2022 - nature.com
Many crowded biomolecular structures in cells and tissues are inaccessible to labelling
antibodies. To understand how proteins within these structures are arranged with nanoscale …

Atlas of Plasmodium falciparum intraerythrocytic development using expansion microscopy

B Liffner, AKC Diaz, J Blauwkamp, D Anaguano… - Elife, 2023 - elifesciences.org
Apicomplexan parasites exhibit tremendous diversity in much of their fundamental cell
biology, but study of these organisms using light microscopy is often hindered by their small …