Experience and memory of environmental stimuli that indicate future stress can prepare
(prime) organismic stress responses even in species lacking a nervous system. The process …

The interaction of bacteria with surfaces has important implications in a range of areas,
including bioenergy, biofouling, biofilm formation, and the infection of plants and animals …

We introduce BacDrop, a highly scalable technology for bacterial single-cell RNA
sequencing that has overcome many challenges hindering the development of scRNA-seq …

Persisters are a subpopulation of antibiotic-tolerant cells responsible for the recalcitrance of
chronic infections. Our current understanding of persister formation is primarily based on …

Microscopy image analysis is a major bottleneck in quantification of single-cell microscopy
data, typically requiring human oversight and curation, which limit both accuracy and …

Beyond fuelling cellular activities with building blocks and energy, metabolism also
integrates environmental conditions into intracellular signals. The underlying regulatory …

CRISPR-Cas adaptive immune systems in prokaryotes boast a diversity of protein families
and mechanisms of action, where most systems rely on protospacer-adjacent motifs (PAMs) …

Heterologous pathways used in metabolic engineering may produce intermediates toxic to
the cell. Dynamic control of pathway enzymes could prevent the accumulation of these …

Evolutionary novelties have been important in the history of life, but their origins are usually
difficult to examine in detail. We previously described the evolution of a novel trait, aerobic …

Biological systems that perform multiple tasks face a fundamental trade-off: A given
phenotype cannot be optimal at all tasks. Here we ask how trade-offs affect the range of …