Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

Cryo-electron tomography (cryo-ET) allows 3D volumes to be reconstructed from a set of 2D
projection images of a tilted biological sample. It allows densities to be resolved in 3D that …

We present a new approach for macromolecular structure determination from multiple
particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram …

Single-particle cryo-electron tomography is an emerging technique capable of determining
the structure of proteins imaged within the native context of cells at molecular resolution …

Macromolecular complexes are intrinsically flexible and often challenging to purify for
structure determination by single-particle cryo-electron microscopy (cryo-EM). Such …

Precise three-dimensional (3D) atomic structure determination of individual nanocrystals is a
prerequisite for understanding and predicting their physical properties. Nanocrystals from …

Cryo-electron tomography allows the routine visualization of cellular landscapes in three
dimensions at nanometer-range resolutions. When combined with single-particle …

Cryoelectron tomography directly visualizes heterogeneous macromolecular structures in
their native and complex cellular environments. However, existing computer-assisted …

Dynamo is a new software package for subtomogram averaging of cryo Electron
Tomography (cryo-ET) data with three main goals: first, Dynamo allows user-transparent …

The envelope glycoproteins (Env) of human and simian immunodeficiency viruses (HIV and
SIV, respectively) mediate virus binding to the cell surface receptor CD4 on target cells to …