Although fluorescent reporters and biosensors have become indispensable tools in
biological and biomedical fields, fluorescence measurements require external excitation …

Imaging of enzyme activity in living subjects promises many applications in both basic and
translational researches from hel** elucidate the enzyme function and mechanism in …

NK cells eliminate virus-infected and tumor cells by releasing cytotoxic granules containing
granzyme B (GrzB) or by engaging death receptors that initiate caspase cascades. The …

Real-time monitoring of cell apoptosis could provide valuable insights into early detection of
therapy efficiency and evaluation of disease progression. In this work, we designed and …

Cellular signal transduction is predominantly based on protein interactions and their post-
translational modifications, which enable a fast response to input signals. Owing to …

All systems GO! An intracellular protease sensor is based on the covalent conjugate of
graphene oxide and peptide substrates with fluorophore labels. The conjugate can be …

Bioluminescence imaging (BLI) exploits the light-emitting properties of luciferase enzymes
for monitoring cells and biomolecular processes in living subjects. Luciferases can be …

Recent progress of bioanalytical techniques has accelerated the deep understanding of
cellular states and development of novel drugs as well as medical diagnosis. Particularly …

A new enzymatic protein ligation tool, sortase, has recently emerged from Gram‐positive
bacteria. This article outlines the technique, sortase‐mediated ligation, and its applications …

It has been estimated that over a billion dollars in resources can be consumed to obtain
clinical approval, and only a few new chemical entities are approved by the US Food and …