As important genome editing tools, CRISPR/Cas systems, especially those based on type II
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …

Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …

CRISPR/Cas9 is an effective gene editing tool with broad applications for the prevention or
treatment of numerous diseases. It depends on CRISPR (clustered regularly interspaced …

The term 'clustered regularly interspaced short palindromic repeats'(CRISPR) has recently
become synonymous with the genome-editing revolution. The RNA-guided endonuclease …

The base‐editing technique using CRISPR/nC as9 (Cas9 nickase) or dC as9 (deactivated
Cas9) fused with cytidine deaminase is a powerful tool to create point mutations. In this …

With developments in synthetic biology,“engineering biology” has emerged through
standardization and platformization based on hierarchical, orthogonal, and modularized …

CRISPR-Cas9 has been demonstrated as a transformative genome engineering tool for
many eukaryotic organisms; however, its utilization in bacteria remains limited and …

Solventogenic clostridia are important industrial microorganisms that produce various
chemicals and fuels. Effective genetic tools would facilitate physiological studies aimed both …

Pseudomonas putida is a microbial chassis of huge potential for industrial and
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …

Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome
engineering, while also changing the speed and efficiency of metabolic engineering in …