CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

All organisms possess molecular mechanisms that govern DNA repair and associated DNA
damage response (DDR) processes. Owing to their relevance to human disease, most …

SLC7A11-mediated cystine uptake suppresses ferroptosis yet promotes cell death under
glucose starvation; the nature of the latter cell death remains unknown. Here we show that …

CRISPR loss of function screens are powerful tools to interrogate biology but exhibit a
number of biases and artifacts that can confound the results. Here, we introduce Chronos …

Genetic interactions mediate the emergence of phenotype from genotype, but technologies
for combinatorial genetic perturbation in mammalian cells are challenging to scale. Here, we …

The genetic circuits that allow cancer cells to evade destruction by the host immune system
remain poorly understood,–. Here, to identify a phenotypically robust core set of genes and …

Covalent DNA–protein cross-links (DPCs) are toxic DNA lesions that block replication and
require repair by multiple pathways. Whether transcription blockage contributes to the …

Progressive multiple sclerosis (MS) is characterized by unrelenting neurodegeneration,
which causes cumulative disability and is refractory to current treatments. Drug development …

The response to DNA damage is critical for cellular homeostasis, tumor suppression,
immunity, and gametogenesis. In order to provide an unbiased and global view of the DNA …

Abstract 53BP1 is a chromatin-binding protein that regulates the repair of DNA double-
strand breaks by suppressing the nucleolytic resection of DNA termini,. This function of …