Measuring NDC80 binding reveals the molecular basis of tension-dependent kinetochore-microtubule attachments

TY Yoo, JM Choi, W Conway, CH Yu, RV Pappu… - Elife, 2018‏ - elifesciences.org
Proper kinetochore-microtubule attachments, mediated by the NDC80 complex, are required
for error-free chromosome segregation. Erroneous attachments are corrected by the tension …

Develo** and testing a Bayesian analysis of fluorescence lifetime measurements

B Kaye, PJ Foster, TY Yoo, DJ Needleman - PLoS one, 2017‏ - journals.plos.org
FRET measurements can provide dynamic spatial information on length scales smaller than
the diffraction limit of light. Several methods exist to measure FRET between fluorophores …

Automated fluorescence lifetime imaging high-content analysis of Förster resonance energy transfer between endogenously labeled kinetochore proteins in live …

W Guo, S Kumar, F Görlitz, E Garcia… - SLAS …, 2019‏ - journals.sagepub.com
We describe an open-source automated multiwell plate fluorescence lifetime imaging (FLIM)
methodology to read out Förster resonance energy transfer (FRET) between fluorescent …

Using FLIM-FRET for characterizing spatial interactions in the spindle

SC Ems-McClung, CE Walczak - Mitosis: Methods and Protocols, 2022‏ - Springer
Proper spindle assembly and the attachment of chromosomes to the spindle are key for the
accurate segregation of chromosomes to daughter cells. Errors in these processes can lead …

FLIM, FRET and high content analysis

E Garcia, W Guo, S Kumar, F Görlitz… - … Microscopy in the …, 2020‏ - spiedigitallibrary.org
Fluorescence lifetime imaging (FLIM) provides a means to contrast different molecular
species and to map variations in the local fluorophore molecular environment, including to …

[ספר][B] Cell Mechanics and Tumor Development

RL Huston - 2020‏ - books.google.com
The focus of this book is on centrioles—small organelles adjacent to the nucleus in all
human and animal (eucaryotic) cells. It provides the findings and critical analyses of over …

[ספר][B] Biophysics of Kinetochore-Microtubule Interaction: Quantitative Measurement of NDC80 Binding in Cell Using FLIM-FRET

TY Yoo - 2017‏ - search.proquest.com
Chromosome motion during cell division is driven by coupling the dynamic ends of
microtubules to the kinetochore. Erroneous kinetochore-microtubule attachments frequently …