Single-cell sequencing-based technologies will revolutionize whole-organism science

E Shapiro, T Biezuner, S Linnarsson - Nature Reviews Genetics, 2013 - nature.com
The unabated progress in next-generation sequencing technologies is fostering a wave of
new genomics, epigenomics, transcriptomics and proteomics technologies. These …

The clockwork embryo: mechanisms regulating developmental rate

M Diaz-Cuadros, O Pourquié - Annual Review of Genetics, 2023 - annualreviews.org
Organismal development requires the reproducible unfolding of an ordered sequence of
discrete steps (cell fate determination, migration, tissue folding, etc.) in both time and space …

Deep learning in label-free cell classification

CL Chen, A Mahjoubfar, LC Tai, IK Blaby, A Huang… - Scientific reports, 2016 - nature.com
Label-free cell analysis is essential to personalized genomics, cancer diagnostics, and drug
development as it avoids adverse effects of staining reagents on cellular viability and cell …

Metabolic regulation of species-specific developmental rates

M Diaz-Cuadros, TP Miettinen, OS Skinner, D Sheedy… - Nature, 2023 - nature.com
Animals display substantial inter-species variation in the rate of embryonic development
despite a broad conservation of the overall sequence of developmental events. Differences …

Single mammalian cells compensate for differences in cellular volume and DNA copy number through independent global transcriptional mechanisms

O Padovan-Merhar, GP Nair, AG Biaesch, A Mayer… - Molecular cell, 2015 - cell.com
Individual mammalian cells exhibit large variability in cellular volume, even with the same
absolute DNA content, and so must compensate for differences in DNA concentration in …

Single-cell entropy for accurate estimation of differentiation potency from a cell's transcriptome

AE Teschendorff, T Enver - Nature communications, 2017 - nature.com
The ability to quantify differentiation potential of single cells is a task of critical importance.
Here we demonstrate, using over 7,000 single-cell RNA-Seq profiles, that differentiation …

The mitophagy receptor BNIP3 is critical for the regulation of metabolic homeostasis and mitochondrial function in the nucleus pulposus cells of the intervertebral disc

V Madhu, M Hernandez-Meadows, PK Boneski, Y Qiu… - Autophagy, 2023 - Taylor & Francis
The contribution of mitochondria to the metabolic function of hypoxic NP cells has been
overlooked. We have shown that NP cells contain networked mitochondria and that …

Mitochondrial levels determine variability in cell death by modulating apoptotic gene expression

S Márquez-Jurado, J Díaz-Colunga… - Nature …, 2018 - nature.com
Fractional killing is the main cause of tumour resistance to chemotherapy. This phenomenon
is observed even in genetically identical cancer cells in homogeneous microenvironments …

Mitochondrial heterogeneity

J Aryaman, IG Johnston, NS Jones - Frontiers in genetics, 2019 - frontiersin.org
Cell-to-cell heterogeneity drives a range of (patho) physiologically important phenomena,
such as cell fate and chemotherapeutic resistance. The role of metabolism, and particularly …

Mitochondrial function and cell size: an allometric relationship

TP Miettinen, M Björklund - Trends in cell biology, 2017 - cell.com
Allometric scaling of metabolic rate results in lower total mitochondrial oxygen consumption
with increasing organismal size. This is considered a universal law in biology. Here, we …