Immobilized enzymes: understanding enzyme–surface interactions at the molecular level

M Hoarau, S Badieyan, ENG Marsh - Organic & Biomolecular …, 2017 - pubs.rsc.org
Enzymes immobilized on solid supports have important and industrial and medical
applications. However, their uses are limited by the significant reductions in activity and …

Engineering and characterization of peptides and proteins at surfaces and interfaces: a case study in surface-sensitive vibrational spectroscopy

B Ding, J Jasensky, Y Li, Z Chen - Accounts of chemical research, 2016 - ACS Publications
Conspectus Understanding molecular structures of interfacial peptides and proteins impacts
many research fields by guiding the advancement of biocompatible materials, new and …

Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

JM Bolivar, I Eisl, B Nidetzky - Catalysis Today, 2016 - Elsevier
Like in chemical catalysis, there is a clear trend in biocatalysis to carry out synthetic
transformations at the manufacturing scale heterogeneously catalyzed. Recycling of …

Studying polymer surfaces and interfaces with sum frequency generation vibrational spectroscopy

X Lu, C Zhang, N Ulrich, M **ao, YH Ma… - Analytical …, 2017 - ACS Publications
Usually a surface or an interface is well-defined with little diffusion from one side of the
surface or interface to the other, with the length scale being on the order of one to several …

Electric fields control the orientation of peptides irreversibly immobilized on radical-functionalized surfaces

LJ Martin, B Akhavan, MMM Bilek - Nature communications, 2018 - nature.com
Surface functionalization of an implantable device with bioactive molecules can overcome
adverse biological responses by promoting specific local tissue integration. Bioactive …

Probing orientations and conformations of peptides and proteins at buried interfaces

W Guo, T Lu, Z Gandhi, Z Chen - The Journal of Physical …, 2021 - ACS Publications
Molecular structures of peptides/proteins at interfaces determine their interfacial properties,
which play important roles in many applications. It is difficult to probe interfacial …

Molecular structure of the surface-immobilized super uranyl binding protein

W Guo, X Zou, H Jiang, KJ Koebke… - The Journal of …, 2021 - ACS Publications
Recently, a super uranyl binding protein (SUP) was developed, which exhibits excellent
sensitivity/selectivity to bind uranyl ions. It can be immobilized onto a surface in sensing …

Chiral sum frequency generation spectroscopy detects double-helix dna at interfaces

EA Perets, KB Olesen, ECY Yan - Langmuir, 2022 - ACS Publications
Many DNA-based technologies involve the immobilization of DNA and therefore require a
fundamental understanding of the DNA structure–function relationship at interfaces. We …

Molecular mechanisms of interactions between monolayered transition metal dichalcogenides and biological molecules

M **ao, S Wei, J Chen, J Tian… - Journal of the …, 2019 - ACS Publications
Single layered two-dimensional (2D) materials such as transition metal dichalcogenides
(TMDs) show great potential in many microelectronic or nanoelectronic applications. For …

Early sum frequency generation vibrational spectroscopic studies on peptides and proteins at interfaces

Z Chen - Biointerphases, 2022 - pubs.aip.org
This paper summarizes the early research results on studying proteins and peptides at
interfaces using sum frequency generation (SFG) vibrational spectroscopy. SFG studies in …