Microorganisms are remarkable chemists, with enzymes as their tools for executing multi-
step syntheses to yield myriad natural products. Microbial synthetic aptitudes are illustrated …

Aminoacyl-tRNAs were long thought to be involved solely in ribosome-dependent protein
synthesis and essential primary metabolism processes, such as targeted protein …

Covering: Up to mid-2019 Cyclodipeptide synthases (CDPSs) catalyse the formation of
cyclodipeptides using aminoacylated-tRNA as substrates. The recent characterization of …

Directed evolution is a powerful approach to engineer enzymes via iterative creation and
screening of variant libraries. However, assay development for high-throughput mutant …

Modified cyclic dipeptides represent a widespread class of secondary metabolites with
diverse pharmacological activities, including antibacterial, antifungal, and antitumor. Here …

Cyclodipeptide synthases (CDPSs) can catalyze the formation of two successive peptide
bonds by hijacking aminoacyl-tRNAs from the ribosomal machinery resulting in …

Cyclodipeptide synthases (CDPSs) use as substrates two amino acids activated as
aminoacyl-tRNAs to synthesize cyclodipeptides in secondary metabolites biosynthetic …

Cyclodipeptide synthases (CDPSs) constitute a family of peptide bond–forming enzymes
that use aminoacyl-tRNAs for the synthesis of cyclodipeptides. Here, we describe the activity …

Transfer RNAs (tRNAs) are the macromolecules that transfer activated amino acids from
aminoacyl‐tRNA synthetases to the ribosome, where they are used for the mRNA guided …

Class I lantibiotic dehydratases dehydrate selected Ser/Thr residues of a precursor peptide.
Recent studies demonstrated the requirement of glutamyl-tRNA Glu for Ser/Thr activation by …