Highlights•Harnessing cellular processes to produce valuable metabolic products is of
growing economic importance.•However, biological complexity necessitates many attempts …

Biotechnological production of phenolic acids is attracting increased interest due to their
superior antioxidant activity, as well as other antimicrobial, dietary, and health benefits. As …

Genetic regulatory proteins inducible by small molecules are useful synthetic biology tools
as sensors and switches. Bacterial allosteric transcription factors (aTFs) are a major class of …

Transition metals function as structural and catalytic cofactors for a large diversity of proteins
and enzymes that collectively comprise the metalloproteome. Metallostasis considers all …

Inducible gene expression systems are vital tools for the advancement of synthetic biology.
Their application as genetically encoded biosensors has the potential to contribute to …

Monitoring cellular behavior and eventually properly adapting cellular processes is key to
handle the enormous complexity of today's metabolic engineering questions. Hence …

Abstract Within the Design-Build-Test Cycle for strain engineering, rapid product detection
and selection strategies remain challenging and limit overall throughput. Here we …

Highlights•Transcription factor-based biosensors are powerful analytical synthetic biology
tools.•Biosensors can be used for detection, metabolic engineering, and protein …

ω-Transaminases (ω-TA) are attractive biocatalysts for the production of chiral amines from
prochiral ketones via asymmetric synthesis. However, the substrate scope of ω-TAs is …

Allosteric transcription factors (aTF) regulate gene expression through conformational
changes induced by small molecule binding. Although widely used as biosensors, aTFs …