Dynamic nuclear polarization for sensitivity enhancement in biomolecular solid-state NMR

T Biedenbänder, V Aladin, S Saeidpour… - Chemical …, 2022 - ACS Publications
Solid-state NMR with magic-angle spinning (MAS) is an important method in structural
biology. While NMR can provide invaluable information about local geometry on an atomic …

[HTML][HTML] Dynamic nuclear polarization for sensitivity enhancement in modern solid-state NMR

ASL Thankamony, JJ Wittmann, M Kaushik… - Progress in nuclear …, 2017 - Elsevier
The field of dynamic nuclear polarization has undergone tremendous developments and
diversification since its inception more than 6 decades ago. In this review we provide an in …

Biomolecular and biological applications of solid-state NMR with dynamic nuclear polarization enhancement

WY Chow, G De Paëpe, S Hediger - Chemical Reviews, 2022 - ACS Publications
Solid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables the
investigation of biological systems within their native context, such as lipid membranes, viral …

Highly efficient, water‐soluble polarizing agents for dynamic nuclear polarization at high frequency

C Sauvée, M Rosay, G Casano… - Angewandte Chemie …, 2013 - Wiley Online Library
Dynamic nuclear polarization coupled to solid-state NMR spectroscopy (DNP/ssNMR) is
emerging as a very powerful technique to overcome the limitations resulting from the …

Facing and overcoming sensitivity challenges in biomolecular NMR spectroscopy

JH Ardenkjaer‐Larsen, GS Boebinger… - Angewandte Chemie …, 2015 - Wiley Online Library
In the Spring of 2013, NMR spectroscopists convened at the Weizmann Institute in Israel to
brainstorm on approaches to improve the sensitivity of NMR experiments, particularly when …

Large molecular weight nitroxide biradicals providing efficient dynamic nuclear polarization at temperatures up to 200 K

A Zagdoun, G Casano, O Ouari… - Journal of the …, 2013 - ACS Publications
A series of seven functionalized nitroxide biradicals (the bTbK biradical and six derivatives)
are investigated as exogenous polarization sources for dynamic nuclear polarization (DNP) …

Rational design of dinitroxide biradicals for efficient cross-effect dynamic nuclear polarization

DJ Kubicki, G Casano, M Schwarzwälder, S Abel… - Chemical …, 2016 - pubs.rsc.org
A series of 37 dinitroxide biradicals have been prepared and their performance studied as
polarizing agents in cross-effect DNP NMR experiments at 9.4 T and 100 K in 1, 1, 2, 2 …

Magic Angle Spinning NMR of Proteins: High-Frequency Dynamic Nuclear Polarization and 1H Detection

Y Su, L Andreas, RG Griffin - Annual review of biochemistry, 2015 - annualreviews.org
Magic angle spinning (MAS) NMR studies of amyloid and membrane proteins and large
macromolecular complexes are an important new approach to structural biology. However …

DNP NMR of biomolecular assemblies

K Jaudzems, T Polenova, G Pintacuda… - Journal of structural …, 2019 - Elsevier
Abstract Dynamic Nuclear Polarization (DNP) is an effective approach to alleviate the
inherently low sensitivity of solid-state NMR (ssNMR) under magic angle spinning (MAS) …

Targeted DNP for biomolecular solid-state NMR

D Gauto, O Dakhlaoui, I Marin-Montesinos… - Chemical …, 2021 - pubs.rsc.org
High-field dynamic nuclear polarization is revolutionizing the scope of solid-state NMR with
new applications in surface chemistry, materials science and structural biology. In this …