Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

INTRODUCTION The eukaryotic nucleus protects the genome and is enclosed by the two
membranes of the nuclear envelope. Nuclear pore complexes (NPCs) perforate the nuclear …

INTRODUCTION The nucleus harbors and protects the genetic information in eukaryotes. It
is surrounded by the nuclear envelope, which separates the nucleoplasm from the cytosol …

Nuclear pore complexes (NPCs) create large conduits for cargo transport between the
nucleus and cytoplasm across the nuclear envelope (NE),–. These multi-megadalton …

The nuclear pore complex (NPC) serves as the sole bidirectional gateway of
macromolecules in and out of the nucleus. Owing to its size and complexity (∼ 1,000 protein …

Nuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of
macromolecules. Here we provide a structure of the isolated yeast NPC in which the inner …

All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

Nuclear pore complexes (NPCs) mediate nucleocytoplasmic exchange. They are
exceptionally large protein complexes that fuse the inner and outer nuclear membranes to …

Integrative structure determination is a powerful approach to modeling the structures of
biological systems based on data produced by multiple experimental and theoretical …