CRISPR technologies for genome, epigenome and transcriptome editing
Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …
understanding of CRISPR biology and its application to genome, epigenome and …
Beyond knockouts: fine‐tuning regulation of gene expression in plants with CRISPR‐Cas‐based promoter editing
The CRISPR‐Cas‐based genome editing field in plants is expanding rapidly. Editing plant
promoters to obtain cis‐regulatory alleles with altered expression levels or patterns of target …
promoters to obtain cis‐regulatory alleles with altered expression levels or patterns of target …
Genotoxic effects of base and prime editing in human hematopoietic stem cells
Base and prime editors (BEs and PEs) may provide more precise genetic engineering than
nuclease-based approaches because they bypass the dependence on DNA double-strand …
nuclease-based approaches because they bypass the dependence on DNA double-strand …
A split and inducible adenine base editor for precise in vivo base editing
DNA base editors use deaminases fused to a programmable DNA-binding protein for
targeted nucleotide conversion. However, the most widely used TadA deaminases lack post …
targeted nucleotide conversion. However, the most widely used TadA deaminases lack post …
In the business of base editors: evolution from bench to bedside
EM Porto, AC Komor - PLoS biology, 2023 - journals.plos.org
With the advent of recombinant DNA technology in the 1970s, the idea of using gene
therapies to treat human genetic diseases captured the interest and imagination of scientists …
therapies to treat human genetic diseases captured the interest and imagination of scientists …
Base editor screens for in situ mutational scanning at scale
A fundamental challenge in biology is understanding the molecular details of protein
function. How mutations alter protein activity, regulation, and response to drugs is of critical …
function. How mutations alter protein activity, regulation, and response to drugs is of critical …
A prime editor mouse to model a broad spectrum of somatic mutations in vivo
ZA Ely, N Mathey-Andrews, S Naranjo, SI Gould… - Nature …, 2024 - nature.com
Genetically engineered mouse models only capture a small fraction of the genetic lesions
that drive human cancer. Current CRISPR–Cas9 models can expand this fraction but are …
that drive human cancer. Current CRISPR–Cas9 models can expand this fraction but are …
Past, present, and future of CRISPR genome editing technologies
Genome editing has been a transformative force in the life sciences and human medicine,
offering unprecedented opportunities to dissect complex biological processes and treat the …
offering unprecedented opportunities to dissect complex biological processes and treat the …
Phage-assisted evolution of highly active cytosine base editors with enhanced selectivity and minimal sequence context preference
TadA-derived cytosine base editors (TadCBEs) enable programmable C• G-to-T• A editing
while retaining the small size, high on-target activity, and low off-target activity of TadA …
while retaining the small size, high on-target activity, and low off-target activity of TadA …
Dimerization of the deaminase domain and locking interactions with Cas9 boost base editing efficiency in ABE8e
CRISPR-based DNA adenine base editors (ABEs) hold remarkable promises to address
human genetic diseases caused by point mutations. ABEs were developed by combining …
human genetic diseases caused by point mutations. ABEs were developed by combining …