Antimicrobial resistance in the environment
This review summarizes selected publications of 2016 with emphasis on occurrence and
treatment of antibiotic resistance genes and bacteria in the aquatic environment and …
treatment of antibiotic resistance genes and bacteria in the aquatic environment and …
[HTML][HTML] Toward a quantitative understanding of antibiotic resistance evolution
M Lukačišinová, T Bollenbach - Current opinion in biotechnology, 2017 - Elsevier
Highlights•Antibiotic resistance depends on general cell physiology and can vary even
among clonal cells.•New techniques enable investigating resistance evolution in temporal or …
among clonal cells.•New techniques enable investigating resistance evolution in temporal or …
CRISPR/Cas9 recombineering‐mediated deep mutational scanning of essential genes in Escherichia coli
Deep mutational scanning can provide significant insights into the function of essential
genes in bacteria. Here, we developed a high‐throughput method for mutating essential …
genes in bacteria. Here, we developed a high‐throughput method for mutating essential …
Chemogenomic model identifies synergistic drug combinations robust to the pathogen microenvironment
M Cokol, C Li, S Chandrasekaran - PLoS computational biology, 2018 - journals.plos.org
Antibiotics need to be effective in diverse environments in vivo. However, the pathogen
microenvironment can have a significant impact on antibiotic potency. Further, antibiotics are …
microenvironment can have a significant impact on antibiotic potency. Further, antibiotics are …
Deep mutational scanning reveals the molecular determinants of RNA polymerase-mediated adaptation and tradeoffs
RNA polymerase (RNAP) is emblematic of complex biological systems that control multiple
traits involving trade-offs such as growth versus maintenance. Laboratory evolution has …
traits involving trade-offs such as growth versus maintenance. Laboratory evolution has …
Synthetic genome defenses against selfish DNA elements stabilize engineered bacteria against evolutionary failure
Mobile genetic elements drive evolution by disrupting genes and rearranging genomes.
Eukaryotes have evolved epigenetic mechanisms, including DNA methylation and RNA …
Eukaryotes have evolved epigenetic mechanisms, including DNA methylation and RNA …
Refactoring the genetic code for increased evolvability
The standard genetic code is robust to mutations during transcription and translation. Point
mutations are likely to be synonymous or to preserve the chemical properties of the original …
mutations are likely to be synonymous or to preserve the chemical properties of the original …
Improving the success and impact of the metabolic engineering design, build, test, learn cycle by addressing proteins of unknown function
LR Jarboe - Current opinion in biotechnology, 2018 - Elsevier
Highlights•Incomplete protein annotation limits our ability to design and engineer
organisms.•Expression libraries, omics data and evolved strain data suggest interesting …
organisms.•Expression libraries, omics data and evolved strain data suggest interesting …
Integrating CRISPR-enabled trackable genome engineering and transcriptomic analysis of global regulators for antibiotic resistance selection and identification in …
It is important to expedite our understanding of antibiotic resistance to address the
increasing numbers of fatalities and environmental pollution due to the emergence of …
increasing numbers of fatalities and environmental pollution due to the emergence of …
Insights on Osmotic Tolerance Mechanisms in Escherichia coli Gained from an rpoC Mutation
An 84 bp in-frame duplication (K370_A396dup) within the rpoC subunit of RNA polymerase
was found in two independent mutants selected during an adaptive laboratory evolution …
was found in two independent mutants selected during an adaptive laboratory evolution …