The mechanisms of integral membrane protein biogenesis

RS Hegde, RJ Keenan - Nature Reviews Molecular Cell Biology, 2022‏ - nature.com
Roughly one quarter of all genes code for integral membrane proteins that are inserted into
the plasma membrane of prokaryotes or the endoplasmic reticulum membrane of …

Molecular machines that facilitate bacterial outer membrane protein biogenesis

MT Doyle, HD Bernstein - Annual Review of Biochemistry, 2024‏ - annualreviews.org
Almost all outer membrane proteins (OMPs) in Gram-negative bacteria contain a β-barrel
domain that spans the outer membrane (OM). To reach the OM, OMPs must be translocated …

Multidrug resistance in bacteria

H Nikaido - Annual review of biochemistry, 2009‏ - annualreviews.org
Large amounts of antibiotics used for human therapy, as well as for farm animals and even
for fish in aquaculture, resulted in the selection of pathogenic bacteria resistant to multiple …

[HTML][HTML] Sec-and Tat-mediated protein secretion across the bacterial cytoplasmic membrane—distinct translocases and mechanisms

P Natale, T Brüser, AJM Driessen - Biochimica et Biophysica Acta (BBA) …, 2008‏ - Elsevier
In bacteria, two major pathways exist to secrete proteins across the cytoplasmic membrane.
The general Secretion route, termed Sec-pathway, catalyzes the transmembrane …

Recombinant protein secretion in Escherichia coli

FJM Mergulhão, DK Summers, GA Monteiro - Biotechnology advances, 2005‏ - Elsevier
The secretory production of recombinant proteins by the Gram-negative bacterium
Escherichia coli has several advantages over intracellular production as inclusion bodies. In …

The ribosome as a platform for co-translational processing, folding and targeting of newly synthesized proteins

G Kramer, D Boehringer, N Ban, B Bukau - Nature structural & …, 2009‏ - nature.com
The early events in the life of newly synthesized proteins in the cellular environment are
remarkably complex. Concurrently with their synthesis by the ribosome, nascent …

The surprising complexity of signal sequences

RS Hegde, HD Bernstein - Trends in biochemical sciences, 2006‏ - cell.com
Most secreted and many membrane proteins contain cleavable N-terminal signal sequences
that mediate their targeting to and translocation across the endoplasmic reticulum or …

Prediction of twin-arginine signal peptides

JD Bendtsen, H Nielsen, D Widdick, T Palmer… - BMC …, 2005‏ - Springer
Background Proteins carrying twin-arginine (Tat) signal peptides are exported into the
periplasmic compartment or extracellular environment independently of the classical Sec …

[HTML][HTML] Structure and function of the molecular chaperone Trigger Factor

A Hoffmann, B Bukau, G Kramer - … et Biophysica Acta (BBA)-Molecular Cell …, 2010‏ - Elsevier
Newly synthesized proteins require the assistance of molecular chaperones to efficiently fold
into functional three-dimensional structures in the crowded environment of the cell. At first …

[HTML][HTML] Protein translocation across the ER membrane

R Zimmermann, S Eyrisch, M Ahmad… - Biochimica et Biophysica …, 2011‏ - Elsevier
Protein translocation into the endoplasmic reticulum (ER) is the first and decisive step in the
biogenesis of most extracellular and many soluble organelle proteins in eukaryotic cells. It is …