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Current challenges and best-practice protocols for microbiome analysis
Analyzing the microbiome of diverse species and environments using next-generation
sequencing techniques has significantly enhanced our understanding on metabolic …
sequencing techniques has significantly enhanced our understanding on metabolic …
Diversity within species: interpreting strains in microbiomes
Studying within-species variation has traditionally been limited to culturable bacterial
isolates and low-resolution microbial community fingerprinting. Metagenomic sequencing …
isolates and low-resolution microbial community fingerprinting. Metagenomic sequencing …
Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis
The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for
decades. However, high-throughput sequencing of the full gene has only recently become a …
decades. However, high-throughput sequencing of the full gene has only recently become a …
Mycobiome diversity: high-throughput sequencing and identification of fungi
Fungi are major ecological players in both terrestrial and aquatic environments by cycling
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …
Environmental DNA metabarcoding: Transforming how we survey animal and plant communities
The genomic revolution has fundamentally changed how we survey biodiversity on earth.
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …
Exact sequence variants should replace operational taxonomic units in marker-gene data analysis
Recent advances have made it possible to analyze high-throughput marker-gene
sequencing data without resorting to the customary construction of molecular operational …
sequencing data without resorting to the customary construction of molecular operational …
Deblur rapidly resolves single-nucleotide community sequence patterns
High-throughput sequencing of 16S ribosomal RNA gene amplicons has facilitated
understanding of complex microbial communities, but the inherent noise in PCR and DNA …
understanding of complex microbial communities, but the inherent noise in PCR and DNA …
[HTML][HTML] Small intestine microbiota regulate host digestive and absorptive adaptive responses to dietary lipids
K Martinez-Guryn, N Hubert, K Frazier, S Urlass… - Cell host & …, 2018 - cell.com
The gut microbiota play important roles in lipid metabolism and absorption. However, the
contribution of the small bowel microbiota of mammals to these diet-microbe interactions …
contribution of the small bowel microbiota of mammals to these diet-microbe interactions …
UNOISE2: improved error-correction for Illumina 16S and ITS amplicon sequencing
RC Edgar - BioRxiv, 2016 - heart.bmj.com
Amplicon sequencing of tags such as 16S and ITS ribosomal RNA is a popular method for
investigating microbial populations. In such experiments, sequence errors caused by PCR …
investigating microbial populations. In such experiments, sequence errors caused by PCR …
New insights into human nostril microbiome from the expanded human oral microbiome database (eHOMD): a resource for the microbiome of the human …
ABSTRACT The expanded Human Oral Microbiome Database (eHOMD) is a
comprehensive microbiome database for sites along the human aerodigestive tract that …
comprehensive microbiome database for sites along the human aerodigestive tract that …