Current challenges and best-practice protocols for microbiome analysis

R Bharti, DG Grimm - Briefings in bioinformatics, 2021 - academic.oup.com
Analyzing the microbiome of diverse species and environments using next-generation
sequencing techniques has significantly enhanced our understanding on metabolic …

Diversity within species: interpreting strains in microbiomes

T Van Rossum, P Ferretti, OM Maistrenko… - Nature Reviews …, 2020 - nature.com
Studying within-species variation has traditionally been limited to culturable bacterial
isolates and low-resolution microbial community fingerprinting. Metagenomic sequencing …

Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis

JS Johnson, DJ Spakowicz, BY Hong… - Nature …, 2019 - nature.com
The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for
decades. However, high-throughput sequencing of the full gene has only recently become a …

Mycobiome diversity: high-throughput sequencing and identification of fungi

RH Nilsson, S Anslan, M Bahram… - Nature Reviews …, 2019 - nature.com
Fungi are major ecological players in both terrestrial and aquatic environments by cycling
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …

Environmental DNA metabarcoding: Transforming how we survey animal and plant communities

K Deiner, HM Bik, E Mächler, M Seymour… - Molecular …, 2017 - Wiley Online Library
The genomic revolution has fundamentally changed how we survey biodiversity on earth.
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …

Exact sequence variants should replace operational taxonomic units in marker-gene data analysis

BJ Callahan, PJ McMurdie, SP Holmes - The ISME journal, 2017 - academic.oup.com
Recent advances have made it possible to analyze high-throughput marker-gene
sequencing data without resorting to the customary construction of molecular operational …

Deblur rapidly resolves single-nucleotide community sequence patterns

A Amir, D McDonald, JA Navas-Molina, E Kopylova… - …, 2017 - journals.asm.org
High-throughput sequencing of 16S ribosomal RNA gene amplicons has facilitated
understanding of complex microbial communities, but the inherent noise in PCR and DNA …

[HTML][HTML] Small intestine microbiota regulate host digestive and absorptive adaptive responses to dietary lipids

K Martinez-Guryn, N Hubert, K Frazier, S Urlass… - Cell host & …, 2018 - cell.com
The gut microbiota play important roles in lipid metabolism and absorption. However, the
contribution of the small bowel microbiota of mammals to these diet-microbe interactions …

UNOISE2: improved error-correction for Illumina 16S and ITS amplicon sequencing

RC Edgar - BioRxiv, 2016 - heart.bmj.com
Amplicon sequencing of tags such as 16S and ITS ribosomal RNA is a popular method for
investigating microbial populations. In such experiments, sequence errors caused by PCR …