Alternative splicing of eukaryotic transcripts is a mechanism that enables cells to generate
vast protein diversity from a limited number of genes. The mechanisms and outcomes of …

The function of neuronal circuits relies on the properties of individual neuronal cells and
their synapses. We propose that a substantial degree of synapse formation and function is …

Computational trajectory inference enables the reconstruction of cell state dynamics from
single-cell RNA sequencing experiments. However, trajectory inference requires that the …

Human stem-cell-derived models provide the promise of accelerating our understanding of
brain disorders, but not knowing whether they possess the ability to mature beyond mid-to …

We have produced gene expression profiles of all 302 neurons of the C. elegans nervous
system that match the single-cell resolution of its anatomy and wiring diagram. Our results …

Alternative splicing is a post-transcriptional regulatory mechanism producing distinct mRNA
molecules from a single pre-mRNA with a prominent role in the development and function of …

Splicing varies across brain regions, but the single-cell resolution of regional variation is
unclear. We present a single-cell investigation of differential isoform expression (DIE) …

Alternative splicing is prevalent in the mammalian brain. To interrogate the functional role of
alternative splicing in neural development, we analyzed purified neural progenitor cells …

The capability to spatially explore RNA biology in formalin-fixed paraffin-embedded (FFPE)
tissues holds transformative potential for histopathology research. Here, we present …

The proper construction of neural circuits requires the generation of diverse cell types, their
distribution to defined regions, and their specific and appropriate wiring. A major objective in …