Photophysical processes in single molecule organic fluorescent probes
EMS Stennett, MA Ciuba, M Levitus - Chemical Society Reviews, 2014 - pubs.rsc.org
The use of organic fluorescent probes in biochemical and biophysical applications of single
molecule spectroscopy and fluorescence microscopy techniques continues to increase. As …
molecule spectroscopy and fluorescence microscopy techniques continues to increase. As …
Understanding FRET as a research tool for cellular studies
Communication of molecular species through dynamic association and/or dissociation at
various cellular sites governs biological functions. Understanding these physiological …
various cellular sites governs biological functions. Understanding these physiological …
Imaging molecular interactions in living cells by FRET microscopy
EA Jares-Erijman, TM Jovin - Current opinion in chemical biology, 2006 - Elsevier
Förster resonance energy transfer (FRET) is applied extensively in all fields of biological
research and technology, generally as a 'nanoruler'with a dynamic range corresponding to …
research and technology, generally as a 'nanoruler'with a dynamic range corresponding to …
Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells
SM Müller, H Galliardt, J Schneider… - Frontiers in plant …, 2013 - frontiersin.org
Förster resonance energy transfer (FRET) describes excitation energy exchange between
two adjacent molecules typically in distances ranging from 2 to 10 nm. The process depends …
two adjacent molecules typically in distances ranging from 2 to 10 nm. The process depends …
Evidence for homodimerization of the c-Fos transcription factor in live cells revealed by fluorescence microscopy and computer modeling
The c-Fos and c-Jun transcription factors, members of the activator protein 1 (AP-1) complex,
form heterodimers and bind to DNA via a basic leucine zipper and regulate the cell cycle …
form heterodimers and bind to DNA via a basic leucine zipper and regulate the cell cycle …
Sex hormone-binding globulin provides a novel entry pathway for estradiol and influences subsequent signaling in lymphocytes via membrane receptor
The complex effects of estradiol on non-reproductive tissues/cells, including lymphoid
tissues and immunocytes, have increasingly been explored. However, the role of sex …
tissues and immunocytes, have increasingly been explored. However, the role of sex …
BH3‐only proteins: The death‐puppeteer's wires
F Ghiotto, F Fais, S Bruno - Cytometry Part A: The Journal of the …, 2010 - Wiley Online Library
Most cell death in vertebrates proceeds through the intrinsic pathway of apoptosis and
results from unregulated increase of mitochondrial membrane permeability. Bcl2‐associated …
results from unregulated increase of mitochondrial membrane permeability. Bcl2‐associated …
High throughput FRET analysis of protein–protein interactions by slide‐based imaging laser scanning cytometry
Laser scanning cytometry (LSC) is a slide‐based technique combining advantages of flow
and image cytometry: automated, high‐throughput detection of optical signals with …
and image cytometry: automated, high‐throughput detection of optical signals with …
A dynamic network of estrogen receptors in murine lymphocytes: fine-tuning the immune response
AE Schneider, É Kárpáti, K Schuszter… - Journal of leukocyte …, 2014 - academic.oup.com
The actual level of circulating estrogen (17β-estradiol, E2) has a serious impact on
regulation of diverse immune cell functions, where their classical cytoplasmic receptors, ERα …
regulation of diverse immune cell functions, where their classical cytoplasmic receptors, ERα …
Measuring FRET in flow cytometry and microscopy
This unit presents protocols describing the measurement of protein associations using FRET
as determined by flow and image cytometry. The proteins under investigation can be labeled …
as determined by flow and image cytometry. The proteins under investigation can be labeled …