Cell-free DNA (cfDNA) in the circulating blood plasma of patients with cancer contains
tumour-derived DNA sequences that can serve as biomarkers for guiding therapy, for the …

Mutations, the fuel of evolution, are first manifested as rare DNA changes within a population
of cells. Although next-generation sequencing (NGS) technologies have revolutionized the …

INTRODUCTION Modern genomics depends on inexpensive short-read sequencing.
Sequenced reads up to a few hundred base pairs in length are computationally mapped to …

Sequencing technology has achieved great advances in the past decade. Studies have
previously shown the quality of specific instruments in controlled conditions. Here, we …

Coexisting microbial cells of the same species often exhibit genetic variation that can affect
phenotypes ranging from nutrient preference to pathogenicity. Here we present inStrain, a …

COVID-19 is caused by the coronavirus SARS-CoV-2, which jumped into the human
population in late 2019 from a currently uncharacterised animal reservoir. Due to this recent …

The gut microbiomes of human populations worldwide have many core microbial species in
common. However, within a species, some strains can show remarkable population …

Targeted PCR amplification and high-throughput sequencing (amplicon sequencing) of 16S
rRNA gene fragments is widely used to profile microbial communities. New long-read …

Unique Molecular Identifiers (UMIs) are random oligonucleotide barcodes that are
increasingly used in high-throughput sequencing experiments. Through a UMI, identical …

The extensive information capacity of DNA, coupled with decreasing costs for DNA synthesis
and sequencing, makes DNA an attractive alternative to traditional data storage. The …