Escherichia coli is by far the most widely used bacterial host for the production of membrane
proteins. Usually, different strains, culture conditions and production regimes are screened …

The last four years have brought exciting progress in membrane protein research. Finally
those many efforts that have been put into expression of eukaryotic membrane proteins are …

We outline a powerful method for the directed evolution of integral membrane proteins in the
inner membrane of Escherichia coli. For a mammalian G protein-coupled receptor, we …

Membrane proteins account for a third of the eukaryotic proteome, but are greatly under-
represented in the Protein Data Bank. Unfortunately, recent technological advances in X-ray …

We recently developed a display method for the directed evolution of integral membrane
proteins in the inner membrane of Escherichia coli for higher expression and stability. For …

The bacterium Escherichia coli is the most widely used expression host for overexpression
trials of membrane proteins. Usually, different strains, culture conditions and expression …

Escherichia coli is the most widely used host for producing membrane proteins. Thus far, to
study the consequences of membrane protein overexpression in E. coli, we have focussed …

Optimization of coding sequences to maximize protein expression yield is often outsourced
to external service providers during commercial gene synthesis and thus unfortunately …

A major barrier to the physical characterization and structure determination of membrane
proteins is low yield in recombinant expression. To address this problem, we have designed …

The efficient production of membrane proteins in bacteria remains a major challenge. In this
work, we sought to identify overexpressed genes that enhance the yields of recombinant …