Comet Assay: Multifaceted Options for Studies of Plant Stress Response

EV Tyutereva, AD Strizhenok, EI Kiseleva… - Horticulturae, 2024 - mdpi.com
Contrarily to chronic stresses, acute (ie, fast and dramatic) changes in environmental factors
like temperature, radiation, concentration of toxic substances, or pathogen attack often lead …

The effect of chitosan–PMAA–NPK nanofertilizer on Pisum sativum plants

NS Khalifa, MN Hasaneen - 3 Biotech, 2018 - Springer
The use of chitosan (CS) as a carrier for slow fertilizer release is a novel trend. The potential
effect of this system in agriculture is still debatable. Here, chitosan (CS) nanoparticles were …

Salicylic acid pre-treatment modulates Pb2+-induced DNA damage vis-à-vis oxidative stress in Allium cepa roots

G Kaur, P Sharma, S Rathee, HP Singh… - … Science and Pollution …, 2021 - Springer
The current study investigated the putative role of salicylic acid (SA) in modulating Pb2+-
induced DNA and oxidative damage in Allium cepa roots. Pb2+ exposure enhanced free …

High-throughput cell analysis based protocol for ploidy determination in anther-derived rice callus

SA Sahoo, Z Jha, SB Verulkar, AK Srivastava… - Plant Cell, Tissue and …, 2019 - Springer
Key message The study provides high-throughput protocol for ploidy determination which
may accelerate the production of double haploids in rice. Abstract Advancements in anther …

Evaluation of irradiation quarantine dose – induced DNA damage in Rhyzopertha dominica and its effect on wheat grains using comet assay

AO Abotaleb, RA Zinhoum, A Gabarty - International Journal of Tropical …, 2021 - Springer
Irradiation is a quarantine treatment option for stored product pests. In the present studies,
we try to investigate the effect of low gamma irradiation doses in the range of 20–300 Gy on …

Salinity stress alerts genome stability and genotoxicity of Ocimum basilicum cultivars.

SS Alotaibi - 2021 - cabidigitallibrary.org
Salinity is an important abiotic stress that greatly influences growth, secondary product
content and genotoxicity in plants. Ocimum basilicum L.(family Lamiaceae) produces a …

A comparative analysis of membrane intactness and genome integrity in pea, barley, and wheat in response to UVC irradiation

M Georgieva, I Nikolova, G Bonchev… - Turkish Journal of …, 2015 - journals.tubitak.gov.tr
The maintenance of plant genome integrity plays a critical function in the processes of DNA
replication, transcription, and repair. Short-wave UV radiation (UVC) is among the most …

[PDF][PDF] Neutral comet profiles: reliable system for analyses of DNA strand breaks distribution

M Georgieva, L Stoilov - Genet Plant Physiol, 2015 - bio21.bas.bg
This paper reports the conversion of 2-dimensional to 3-dimensional comet profiles
produced by different mutagens able to induce DNA double-strand breaks–radiomimetic …

Evaluation of DNA damage in tobacco male meiocytes involved in cytomixis using comet assay

S Mursalimov, A Zagorskaya, E Deineko - Protoplasma, 2018 - Springer
Cytomixis is a process of nuclear migration between plant cells. As a rule, it is detectable in
male meiocytes and gives rise to the cells with micronuclei. Examination of the integrity and …

[PDF][PDF] Identification of Radiation-Processed Edible Grains and Seeds using Single Cell Microgel Electrophoresis Assay: A Comparative Analysis

KA Ahmad, K Ayesha, SM Kashif - researchgate.net
The DNA Comet assay (microgel electrophoresis method) is a recommended technique for
detection of radiation treatment in food containing DNA material. It involves isolating …