Three-dimensional localization of single molecules for super-resolution imaging and single-particle tracking

L von Diezmann, Y Shechtman, WE Moerner - Chemical reviews, 2017 - ACS Publications
Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …

Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges

L Möckl, WE Moerner - Journal of the American Chemical …, 2020 - ACS Publications
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …

The emergence of phase separation as an organizing principle in bacteria

CA Azaldegui, AG Vecchiarelli, JS Biteen - Biophysical journal, 2021 - cell.com
Recent investigations in bacteria suggest that membraneless organelles play a crucial role
in the subcellular organization of bacterial cells. However, the biochemical functions and …

A guide to single-particle tracking

F Simon, LE Weiss, S van Teeffelen - Nature Reviews Methods Primers, 2024 - nature.com
Individual proteins and protein complexes undergo various motion types, including free
diffusion, confinement, subdiffusion and directed motion. Different motion behaviours reflect …

Splitsville: structural and functional insights into the dynamic bacterial Z ring

DP Haeusser, W Margolin - Nature Reviews Microbiology, 2016 - nature.com
Bacteria must divide to increase in number and colonize their niche. Binary fission is the
most widespread means of bacterial cell division, but even this relatively simple mechanism …

Polyphosphate drives bacterial heterochromatin formation

F Beaufay, HM Amemiya, J Guan, J Basalla… - Science …, 2021 - science.org
Heterochromatin is most often associated with eukaryotic organisms. Yet, bacteria also
contain areas with densely protein-occupied chromatin that appear to silence gene …

Tools for the microbiome: nano and beyond

JS Biteen, PC Blainey, ZG Cardon, M Chun… - ACS …, 2016 - ACS Publications
The microbiome presents great opportunities for understanding and improving the world
around us and elucidating the interactions that compose it. The microbiome also poses …

Liquid-liquid phase separation: Unraveling the enigma of biomolecular condensates in microbial cells

Z Gao, W Zhang, R Chang, S Zhang, G Yang… - Frontiers in …, 2021 - frontiersin.org
Numerous examples of microbial phase-separated biomolecular condensates have now
been identified following advances in fluorescence imaging and single molecule microscopy …

BR-bodies provide selectively permeable condensates that stimulate mRNA decay and prevent release of decay intermediates

N Al-Husini, DT Tomares, ZJ Pfaffenberger… - Molecular cell, 2020 - cell.com
Biomolecular condensates play a key role in organizing RNAs and proteins into
membraneless organelles. Bacterial RNP-bodies (BR-bodies) are a biomolecular …

Progress and prospects for small-molecule probes of bacterial imaging

O Kocaoglu, EE Carlson - Nature chemical biology, 2016 - nature.com
Fluorescence microscopy is an essential tool for the exploration of cell growth, division,
transcription and translation in eukaryotes and prokaryotes alike. Despite the rapid …