Three-dimensional localization of single molecules for super-resolution imaging and single-particle tracking
Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …
two imaging modalities that illuminate the properties of cells and materials on spatial scales …
Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges
L Möckl, WE Moerner - Journal of the American Chemical …, 2020 - ACS Publications
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
The emergence of phase separation as an organizing principle in bacteria
Recent investigations in bacteria suggest that membraneless organelles play a crucial role
in the subcellular organization of bacterial cells. However, the biochemical functions and …
in the subcellular organization of bacterial cells. However, the biochemical functions and …
A guide to single-particle tracking
Individual proteins and protein complexes undergo various motion types, including free
diffusion, confinement, subdiffusion and directed motion. Different motion behaviours reflect …
diffusion, confinement, subdiffusion and directed motion. Different motion behaviours reflect …
Splitsville: structural and functional insights into the dynamic bacterial Z ring
DP Haeusser, W Margolin - Nature Reviews Microbiology, 2016 - nature.com
Bacteria must divide to increase in number and colonize their niche. Binary fission is the
most widespread means of bacterial cell division, but even this relatively simple mechanism …
most widespread means of bacterial cell division, but even this relatively simple mechanism …
Polyphosphate drives bacterial heterochromatin formation
Heterochromatin is most often associated with eukaryotic organisms. Yet, bacteria also
contain areas with densely protein-occupied chromatin that appear to silence gene …
contain areas with densely protein-occupied chromatin that appear to silence gene …
Tools for the microbiome: nano and beyond
The microbiome presents great opportunities for understanding and improving the world
around us and elucidating the interactions that compose it. The microbiome also poses …
around us and elucidating the interactions that compose it. The microbiome also poses …
Liquid-liquid phase separation: Unraveling the enigma of biomolecular condensates in microbial cells
Z Gao, W Zhang, R Chang, S Zhang, G Yang… - Frontiers in …, 2021 - frontiersin.org
Numerous examples of microbial phase-separated biomolecular condensates have now
been identified following advances in fluorescence imaging and single molecule microscopy …
been identified following advances in fluorescence imaging and single molecule microscopy …
BR-bodies provide selectively permeable condensates that stimulate mRNA decay and prevent release of decay intermediates
N Al-Husini, DT Tomares, ZJ Pfaffenberger… - Molecular cell, 2020 - cell.com
Biomolecular condensates play a key role in organizing RNAs and proteins into
membraneless organelles. Bacterial RNP-bodies (BR-bodies) are a biomolecular …
membraneless organelles. Bacterial RNP-bodies (BR-bodies) are a biomolecular …
Progress and prospects for small-molecule probes of bacterial imaging
O Kocaoglu, EE Carlson - Nature chemical biology, 2016 - nature.com
Fluorescence microscopy is an essential tool for the exploration of cell growth, division,
transcription and translation in eukaryotes and prokaryotes alike. Despite the rapid …
transcription and translation in eukaryotes and prokaryotes alike. Despite the rapid …