The spliceosome removes introns from messenger RNA precursors (pre-mRNA). Decades
of biochemistry and genetics combined with recent structural studies of the spliceosome …

Recent successes illustrate the role of mass spectrometry-based proteomics as an
indispensable tool for molecular and cellular biology and for the emerging field of systems …

Most cellular processes are carried out by multiprotein complexes. The identification and
analysis of their components provides insight into how the ensemble of expressed proteins …

The recent abundance of genome sequence data has brought an urgent need for systematic
proteomics to decipher the encoded protein networks that dictate cellular function. To date …

Proteomics, the large-scale analysis of proteins, will contribute greatly to our understanding
of gene function in the post-genomic era. Proteomics can be divided into three main …

We describe a rapid, sensitive process for comprehensively identifying proteins in
macromolecular complexes that uses multidimensional liquid chromatography (LC) and …

Proteomics is an increasingly powerful and indispensable technology in molecular cell
biology. It can be used to identify the components of small protein complexes and large …

▪ Abstract A decade after the discovery of electrospray and matrix-assisted laser desorption
ionization (MALDI), methods that finally allowed gentle ionization of large biomolecules …

Genome sequencing provides a wealth of information on predicted gene products (mostly
proteins), but the majority of these have no known function. Two-dimensional gel …

The emergence of proteomics, the large-scale analysis of proteins, has been inspired by the
realization that the final product of a gene is inherently more complex and closer to function …