Modulating biomolecular condensates: a novel approach to drug discovery

DM Mitrea, M Mittasch, BF Gomes, IA Klein… - Nature Reviews Drug …, 2022 - nature.com
In the past decade, membraneless assemblies known as biomolecular condensates have
been reported to play key roles in many cellular functions by compartmentalizing specific …

Physical principles underlying the complex biology of intracellular phase transitions

JM Choi, AS Holehouse… - Annual review of …, 2020 - annualreviews.org
Many biomolecular condensates appear to form via spontaneous or driven processes that
have the hallmarks of intracellular phase transitions. This suggests that a common …

Intermolecular interactions underlie protein/peptide phase separation irrespective of sequence and structure at crowded milieu

M Poudyal, K Patel, L Gadhe, AS Sawner… - Nature …, 2023 - nature.com
Liquid-liquid phase separation (LLPS) has emerged as a crucial biological phenomenon
underlying the sequestration of macromolecules (such as proteins and nucleic acids) into …

Expanding the molecular language of protein liquid–liquid phase separation

S Rekhi, CG Garcia, M Barai, A Rizuan, BS Schuster… - Nature …, 2024 - nature.com
Understanding the relationship between a polypeptide sequence and its phase separation
has important implications for analysing cellular function, treating disease and designing …

Evidence for widespread cytoplasmic structuring into mesoscale condensates

FC Keber, T Nguyen, A Mariossi, CP Brangwynne… - Nature cell …, 2024 - nature.com
Compartmentalization is an essential feature of eukaryotic life and is achieved both via
membrane-bound organelles, such as mitochondria, and membrane-less biomolecular …

Precise prediction of phase-separation key residues by machine learning

J Sun, J Qu, C Zhao, X Zhang, X Liu, J Wang… - Nature …, 2024 - nature.com
Understanding intracellular phase separation is crucial for deciphering transcriptional
control, cell fate transitions, and disease mechanisms. However, the key residues, which …

The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

S Lu, Q Ye, D Singh, Y Cao, JK Diedrich… - Nature …, 2021 - nature.com
The multifunctional nucleocapsid (N) protein in SARS-CoV-2 binds the~ 30 kb viral RNA
genome to aid its packaging into the 80–90 nm membrane-enveloped virion. The N protein …

Prediction of liquid–liquid phase separating proteins using machine learning

X Chu, T Sun, Q Li, Y Xu, Z Zhang, L Lai, J Pei - BMC bioinformatics, 2022 - Springer
Background The liquid–liquid phase separation (LLPS) of biomolecules in cell underpins
the formation of membraneless organelles, which are the condensates of protein, nucleic …

Molecular and environmental determinants of biomolecular condensate formation

JA Villegas, M Heidenreich, ED Levy - Nature chemical biology, 2022 - nature.com
Biomolecular condensate formation has been implicated in a host of biological processes
and has found relevance in biology and disease. Understanding the physical principles and …

Properties of stress granule and P-body proteomes

JY Youn, BJA Dyakov, J Zhang, JDR Knight… - Molecular cell, 2019 - cell.com
Stress granules and P-bodies are cytosolic biomolecular condensates that dynamically form
by the phase separation of RNAs and proteins. They participate in translational control and …