Mycobiome diversity: high-throughput sequencing and identification of fungi

RH Nilsson, S Anslan, M Bahram… - Nature Reviews …, 2019 - nature.com
Fungi are major ecological players in both terrestrial and aquatic environments by cycling
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …

From hairballs to hypotheses–biological insights from microbial networks

L Röttjers, K Faust - FEMS microbiology reviews, 2018 - academic.oup.com
Microbial networks are an increasingly popular tool to investigate microbial community
structure, as they integrate multiple types of information and may represent systems-level …

High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution

BJ Callahan, J Wong, C Heiner, S Oh… - Nucleic acids …, 2019 - academic.oup.com
Targeted PCR amplification and high-throughput sequencing (amplicon sequencing) of 16S
rRNA gene fragments is widely used to profile microbial communities. New long-read …

Perspectives and benefits of high-throughput long-read sequencing in microbial ecology

L Tedersoo, M Albertsen, S Anslan… - Applied and …, 2021 - Am Soc Microbiol
Short-read, high-throughput sequencing (HTS) methods have yielded numerous important
insights into microbial ecology and function. Yet, in many instances short-read HTS …

The not-so-sterile womb: evidence that the human fetus is exposed to bacteria prior to birth

LF Stinson, MC Boyce, MS Payne… - Frontiers in microbiology, 2019 - frontiersin.org
The human microbiome includes trillions of bacteria, many of which play a vital role in host
physiology. Numerous studies have now detected bacterial DNA in first-pass meconium and …

High-accuracy long-read amplicon sequences using unique molecular identifiers with Nanopore or PacBio sequencing

SM Karst, RM Ziels, RH Kirkegaard, EA Sørensen… - Nature …, 2021 - nature.com
High-throughput amplicon sequencing of large genomic regions remains challenging for
short-read technologies. Here, we report a high-throughput amplicon sequencing approach …

Interpretations of environmental microbial community studies are biased by the selected 16S rRNA (gene) amplicon sequencing pipeline

D Straub, N Blackwell, A Langarica-Fuentes… - Frontiers in …, 2020 - frontiersin.org
One of the major methods to identify microbial community composition, to unravel microbial
population dynamics, and to explore microbial diversity in environmental samples is high …

PacBio metabarcoding of Fungi and other eukaryotes: errors, biases and perspectives

L Tedersoo, A Tooming‐Klunderud… - New Phytologist, 2018 - Wiley Online Library
Second‐generation, high‐throughput sequencing methods have greatly improved our
understanding of the ecology of soil microorganisms, yet the short barcodes (< 500 bp) …

Fusarium head blight from a microbiome perspective

I Karlsson, P Persson, H Friberg - Frontiers in Microbiology, 2021 - frontiersin.org
The fungal genus Fusarium causes several diseases in cereals, including Fusarium head
blight (FHB). A number of Fusarium species are involved in disease development and …

Soil microbiota manipulation and its role in suppressing soil-borne plant pathogens in organic farming systems under the light of microbiome-assisted strategies

U De Corato - Chemical and Biological Technologies in Agriculture, 2020 - Springer
Soil microbiota plays a key role in suppressing soil-borne plant pathogens improving the
natural soil suppressiveness. Microbiome disturbance triggers specific perturbation to …