Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …

Super-resolution microscopy has become an increasingly popular and robust tool across the
life sciences to study minute cellular structures and processes. However, with the increasing …

Anyone who has used a light microscope has wished that its resolution could be a little
better. Now, after centuries of gradual improvements, fluorescence microscopy has made a …

Two-photon excitation of fluorescent proteins is an attractive approach for imaging living
systems. Today researchers are eager to know which proteins are the brightest and what the …

Abstract Extending three-dimensional (3D) single-molecule localization microscopy away
from the coverslip and into thicker specimens will greatly broaden its biological utility …

We demonstrate single-molecule fluorescence imaging beyond the optical diffraction limit in
3 dimensions with a wide-field microscope that exhibits a double-helix point spread function …

Understanding molecular-scale architecture of cells requires determination of 3D locations
of specific proteins with accuracy matching their nanometer-length scale. Existing electron …

Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical
neuronal wiring maps as well as information on how neurons in these networks engage in …

The mathematical theory of compressed sensing (CS) asserts that one can acquire signals
from measurements whose rate is much lower than the total bandwidth. Whereas the CS …

We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick
specimens (50–150 μm), by coupling far-field individual molecule localization with selective …