Decoding the spermatogenesis program: new insights from transcriptomic analyses
Spermatogenesis is a complex differentiation process coordinated spatiotemporally across
and along seminiferous tubules. Cellular heterogeneity has made it challenging to obtain …
and along seminiferous tubules. Cellular heterogeneity has made it challenging to obtain …
Molecular regulation of spermatogonial stem cell renewal and differentiation
The intricate molecular and cellular interactions between spermatogonial stem cells (SSCs)
and their cognate niche form the basis for life-long sperm production. To maintain long-term …
and their cognate niche form the basis for life-long sperm production. To maintain long-term …
Efficient low-cost chromatin profiling with CUT&Tag
Abstract We recently introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an
epigenomic profiling strategy in which antibodies are bound to chromatin proteins in situ in …
epigenomic profiling strategy in which antibodies are bound to chromatin proteins in situ in …
EmptyDrops: distinguishing cells from empty droplets in droplet-based single-cell RNA sequencing data
Droplet-based single-cell RNA sequencing protocols have dramatically increased the
throughput of single-cell transcriptomics studies. A key computational challenge when …
throughput of single-cell transcriptomics studies. A key computational challenge when …
Improved CUT&RUN chromatin profiling tools
Previously, we described a novel alternative to chromatin immunoprecipitation, CUT&RUN,
in which unfixed permeabilized cells are incubated with antibody, followed by binding of a …
in which unfixed permeabilized cells are incubated with antibody, followed by binding of a …
Alternative splicing during mammalian organ development
Alternative splicing (AS) is pervasive in mammalian genomes, yet cross-species
comparisons have been largely restricted to adult tissues and the functionality of most AS …
comparisons have been largely restricted to adult tissues and the functionality of most AS …
The molecular evolution of spermatogenesis across mammals
The testis produces gametes through spermatogenesis and evolves rapidly at both the
morphological and molecular level in mammals,,,,–, probably owing to the evolutionary …
morphological and molecular level in mammals,,,,–, probably owing to the evolutionary …
Cell-fate transition and determination analysis of mouse male germ cells throughout development
Mammalian male germ cell development is a stepwise cell-fate transition process; however,
the full-term developmental profile of male germ cells remains undefined. Here, by …
the full-term developmental profile of male germ cells remains undefined. Here, by …
The XRN1-regulated RNA helicase activity of YTHDC2 ensures mouse fertility independently of m6A recognition
The functional consequence of N 6-methyladenosine (m 6 A) RNA modification is mediated
by" reader" proteins of the YTH family. YTH domain-containing 2 (YTHDC2) is essential for …
by" reader" proteins of the YTH family. YTH domain-containing 2 (YTHDC2) is essential for …
Single-cell RNA sequencing of the cynomolgus macaque testis reveals conserved transcriptional profiles during mammalian spermatogenesis
Spermatogenesis is highly orchestrated and involves the differentiation of diploid
spermatogonia into haploid sperm. The process is driven by spermatogonial stem cells …
spermatogonia into haploid sperm. The process is driven by spermatogonial stem cells …